T ogether with bones, cartilage is one of the main supporting tissues in animals. Cartilage engineering is a strategic experimental goal for the treatment of multiple joint diseases. There are three types of adult cartilage distributed in many areas of the skeleton, particularly in joints and where pliable support is useful, as in the ribs, ears, and nose. The striations are the result of the precise arrangement of actin and myosin into repeating units called sarcomeres that create a characteristic pattern of transverse bands. To maximize the force that human skeletal muscle can produce, the muscle cells. Hyaline cartilage definition of hyaline cartilage by. Fresh hyaline cartilage is bluishwhite and translucent. Is it hyaline, fibrocartilage, or elastic cartilage. It is the ability to distinguish fibers in routine histology preparations that is central to distinguishing fibrocartilage from hyaline cartilage. The lack of blood vessels in hyaline cartilage means that nutrients and wastes must diffuse through the tissue, thus limiting the thickness of the hyaline cartilage.
Hyaline cartilage may be seen slide on several other slides, notably developing cartilage bone. After 12 weeks in culture, the neocartilage shared similarities with native cartilage with regard to chondrocyte phenotype, matrix distribution and the ultrastructure of collagen fibrils. In histopathological medical usage, a hyaline substance appears glassy and pink after being stained with haematoxylin and eosinusually it is an acellular, proteinaceous material. Thus we see that cartilage can grow both ways from the surface perichondrium and from within. Cartilage mucopolysaccharides 261 table 1 the staining properties of hyaline cartiluge untreated methylation deesterification sulfation lacunar capsule. Cartilage is a semirigid structure that maintains the shape of several organs, covers the surface of bones in the joints, and is. Fibro is found in invertebral discs, joint capsules, ligaments.
The mature hyaline cartilage is easily differentiated by its bluepurple appearance, glassy extracellular matrix, and clusters of cells. Oct 28, 2014 lecture 1 histology of cartilage and bonerev1 readonly. Not present over articular cartilage produce proteins, hyaluronic acid lubricant, nutrition for cartilage positive for vcam1, vimentin. Hyaline cartilage engineered by chondrocytes in pellet. Components of romanovsky stain are methylene blue and eosin. It tends to stain more blue than other kinds of connective tissue however, remember that color should never be the main cue you use to identify a tissue. Hyaline cartilage has a pearly bluishwhite tinge and under a lowpower microscope the matrix appears amorphous and translucent semitransparent as in figure 4.
Lecture 1 histology of cartilage and bonerev1 readonly. Click on the button below the histology image and you will hear me give a brief description of the histology slide. Hyaline cartilage is identified by its bluepurple appearance and by its isogenous groups of chondrocytes. This strong cartilage is much more thickly embedded with collagen than is hyaline cartilage. The appearance of the cartilage in this preparation a blue green colour will depend on the method used to show tissue components other than elastic fibres.
It is also most commonly found in the ribs, nose, larynx, and trachea. Staining properties of hyaline cartilage deep blue. A histological and ultrastructural study of femoral head. See more ideas about hyaline cartilage, anatomy and physiology and histology slides. In adults, hyaline cartilage is located in the articular surfaces of. Fresh hyaline cartilage has a translucent appearance, hence the term. Learn vocabulary, terms, and more with flashcards, games, and other study tools. Immunohistochemically, fibrocartilage is typically. Chondrocytes may lie singly or in pairs, but most often they form short rows between dense bundles of collagen fibres. Hyaline cartilage is characterized by chondroblasts within a lacuna and a large amount of white space. Compact bone diaphysis of a long bone ground, unstained and schmorl stained. This function mainly relies on the extracellular matrix. In handbook of histology methods for bone and cartilage, y. In contrast to other cartilage types, collagen type i is dominant in fibrous cartilagedominant in fibrous cartilage.
Hyaline cartilage an overview sciencedirect topics. Trachea hyaline cartilage and pseudostratified ciliated. See your book and the text powerpoints for more information on long bone growth. Comprehensive and uptodate, the handbook of histology methods for bone and cartilage offers researchers a practical guide to all the major histological and histomorphometrical methods of bone and cartilage analysisa gold mine of detailed techniques for the preparation of tissue from collection through fixation, embedding, sectioning, and. An example is hyaline cartilage, a transparent, glossy articular. In the embryo, it serves as a temporary skeleton until it is gradually replaced by bone. In adults, hyaline cartilage is located in the articular surfaces of movable joints, in the walls of the respiratory tracts nose, larynx, trachea, and bronchi, in the costal cartilages, and in the epiphyseal.
The exact position at which the chondrogenic layer becomes cartilage proper is difficult to pinpoint, however, because the two layers blend imperceptibly with each other. May 11, 2020 hyaline cartilage is the most common of the three types of carticlage. Hyaline cartilage is the glasslike hyaline but translucent cartilage found on many joint. Both mutant and control cartilage specimens were taken from the same position in the margin of the femoral head, because the patients who had undergone total hip replacement were suffering from severe osteoarthritis, according to the radiographic appearance, as a result of which, the cartilage in the loadbearing surface was almost completely. A comprehensive, fun and entertaining site devoted exclusively to histology. Note the numerous chondrocytes in this image, each located within lacunae and surrounded by the cartilage they have produced. Hyaline and elastic cartilage are surrounded by a connective tissue capsule called. Cartilage is a flexible connective tissue found in many parts of the body. Cartilage is a resilient and smooth elastic tissue, a rubberlike padding that covers and protects the ends of long bones at the joints, and is a structural component of the rib. In the fetus, a provisional skeleton is created by hyaline cartilage, which during the process of endochondral bone formation is replaced by bone. Alcian blue colloidal iron aldehyde fuchsin azure a 2. Test your knowledge and try and answer these questions. Take up this short and easy cartilage quiz and see how much you know about the cartilage in our bodies.
The detailed methods include techniques for bone labeling, bone sectioning, histological analysis, histomorphometric analysis, radiological examination, and. Hyaline cartilagemed studentanatomy and physiologyhuman anatomyschool stuffnursingmedicinesciencehealth. Hyaline cartilage made of a homogeneous matrix 6080% water and type ii collagen. Comprehensive and uptodate, the handbook of histology methods for bone and cartilage offers researchers a practical guide to all the major histological and histomorphometrical methods of bone and cartilage analysisa gold mine of detailed. The photomicrographs show the main features of b hyaline cartilage, c fibrocartilage. The three types of cartilage there are three types of cartilage. Chondroblasts in existing cartilage divide and form small groups of cells isogenous groups which produce matrix to become separated from each other by a thin partition of matrix. See more ideas about histology slides, white matter and human anatomy and physiology. This rapidly growing tissue forms the majority of the fetal skeleton and remains into adulthood as the smooth joint surfaces at the. The composition and histological organization of bone are welldescribed in most histology books. It is designed to be an experimental guide for personnel who work in the areas of basic and clinical bone and cartilage, orthopedic, or dental research. In hyaline cartilage, fibers are not normally visible in routine sections, for fiber staining is masked by the rest of the matrix stockwell, 1979. Hyaline cartilage is the glasslike hyaline but translucent cartilage found on many joint surfaces. In the picture on the left, brown fat is at the top left and white fat at bottom right.
Cartilage is a resilient and smooth elastic tissue, a rubberlike padding that covers and protects the ends of long bones at the joints, and is a structural component of the rib cage, the ear, the nose, the bronchial tubes, the intervertebral discs, and many other body components. Martin have assembled an extensive collection of the major methods for studying the histology and histomorphometry of bone. Elastic cartilage has abundant elastic fibers in addition to collagen, making the matrix. Cartilage support of other tissues throughout the respiratory system is also prominent. Also at ventral ends of ribs and as articular cartilage on the bone surfaces. Hyaline cartilage histology slides cell biology organic chemistry bones and muscles muscle tissue radiology ribs hyaline cartilage hyaline cartilage has a matrix composed of type ii collagen and chondromucoprotein, a copolymer of chondroitin sulfates a and.
Handbook of histology methods for bone and cartilage is an outgrowth of the editors own quest for information on bone and cartilage histology and histomorphometry. No blood supply, no lymphatics, no innervation thickest at periphery. Fibro is found in invertebral discs, joint capsules, ligaments elastic is found in the external ear. Within the outer ear, it provides the skeletal basis of the pinna, as well as the. Hyaline cartilage is characteristically imaged as an anechoic, homogeneous.
In its fresh state, it is homogeneous and semitransparent. Fibrocartilage is a support and binding tissue with strong shockabsorbing capabilities. Although the matrix appears blue green, the typical organisation of cartilage is readily visible. Some lacunae contain more than one chondrocyte why is this. In this picture also note the blue line drawn to show the two parts separately and single cells in circles. Robert lewis maynard, noel downes, in anatomy and histology of the. In contrast to other cartilage types, collagen type i is dominant in fibrous cartilage. In the picture on the right, white fat is at top left. Hyaline most common, found in the ribs, nose, larynx, trachea. The bar shows the position of the hyaline cartilage. Most of the skeleton is preformed in hyaline cartilage. What do you think the function of the cartilage in this location is. The most striking feature of skeletal muscle fibers is the presence of striations, or lines, visible in longitudinal sections of the muscle.
Hyaline cartilage definition of hyaline cartilage by the. Hyaline cartilage is an important component of the wall of the upper respiratory tract, where it prevents. Under a highpower microscope the matrix can be seen to consist of a dense network of very fine collagen fibrils and fibres embedded in proteoglycan gel williams et al 1995. Based on the process of embryonic chondrogenesis, we hypothesized that cartilage could be engineered by condensing chondrocytes in pellet culture and, in the present study, examined the quality of regenerated cartilage in direct comparison with native cartilage. An example is hyaline cartilage, a transparent, glossy articular joint cartilage. It persists in human adults at the ends of bones in freemoving joints as articular cartilage, at the ends of the ribs, and in the nose, larynx, trachea, and. Ground bone, demineralized bone from human rib, hyaline cartilage, elastic cartilage from epiglotus, fibrocartilage, bone from nasal concha, intramembranous. Elastic is found in the external ear, epiglottis and larynx.
Hls cartilage and bone and bone histogenesis, hyaline. Histology learning system cartilage and bone and bone histogenesis, hyaline cartilage. Immature and mature cartilage forming cells located at articular cartilage regions. Hyaline cartilage figure 72 is the most common and best studied of the three forms.
Hyaline cartilage is a vital component of epiphyseal growth plates, which regulates the growth as well as shape of long bones soon after birth till adolescence. Handbook of histology methods for bone and cartilage. Hyaline cartilage definition is translucent bluishwhite cartilage with the cells embedded in an apparently homogeneous matrix present in joints and respiratory passages and forming most of the fetal skeleton. It is designed to be an experimental guide for personnel who work in the areas of basic and clinical bone and. Grossly, hyaline cartilage is opaque and flexible think of the sternal cartilage associated with a chicken breast. The hyaline cartilage in the trachea is in the middle of the tracheal wall. Elastic cartilage, sometimes referred to as yellow fibrocartilage, is a type of cartilage that provides both strength and elasticity to certain parts of the body, such as the ears. This is a section of fibrocartilage from the quadriceps tendon near its junction with the patella. Hyaline cartilage is a translucent white type of cartilage present in areas of the body such as the joints.
This site includes histology quizzes, histology games, slides, mnemonics, histology puzzles and tons of information about histology. Hyaline cartilage, the most common type of cartilage, is composed of type ii collagen and chondromucoprotein and often has a glassy appearance. This section comes from cartilage in a developing fetal bone. See more ideas about medical science, anatomy and physiology and histology slides. Cartilage is a resilient connective tissue composed of cells embedded in an extracellular matrix that is gellike and has a rigid consistency. This is a section of hyaline cartilage, the most abundant type of cartilage in the body. At the periphery, young chondrocytes or chondroblasts have an elliptic shape, with their long axis parallel to the surface.
Note the numerous chondrocytes in this image, each located within lacunae and surrounded by. These cells have relatively small nuclei and often. Contains chondrocytes, perichondrium, isogenous groups, territorial matrix, and interterritorial matrix. Only few of the haversian systems are textbook circular.